Fully Automatic Colony Counter

Product Introduction

The HD-C330 Fully Automatic Colony Counter is designed for automatic colony imaging, intelligent colony recognition, colony counting, data analysis, and report output in microbiology laboratories.

The instrument adopts a fully enclosed metal imaging chamber to eliminate interference from ambient light. Its full-mode illumination system integrates fog diffuse reflection lighting, white transparent bottom lighting, and compound suspended dark-field lighting, making it suitable for imaging different types of Petri dishes and microbial culture plates.

The colony counting software is equipped with an intelligent image recognition algorithm, which can process complex colony images, automatically identify colonies, separate connected colonies, remove impurities, and generate reliable statistical results. This helps laboratories reduce manual counting workload, avoid repeated parameter adjustment, and improve the efficiency and consistency of colony analysis.

Working Principle

The HD-C330 captures high-resolution images of culture plates inside a closed imaging chamber. By using different lighting modes, the system creates suitable imaging conditions for various sample types, including pour plates, spread plates, membrane filtration plates, spiral plates, and microbial test films.

After image acquisition, the software analyzes the colony image using intelligent image recognition algorithms. Colonies can be identified and counted based on size, color, shape, area, perimeter, equivalent diameter, and other image features.

For complex samples, the software can automatically separate connected colonies, exclude impurities, count colonies in circular, square, or manually selected regions, and calculate dilution factors automatically. The final results can be stored, printed, or exported for laboratory records.

Main Applications

The HD-C330 Fully Automatic Colony Counter is suitable for colony counting and microbial image analysis in various laboratory workflows.

Typical applications include:

• Food microbiology testing
• Pharmaceutical microbiology testing
• Environmental microbiology testing
• Drinking water and water quality testing
• Clinical microbiology research
• Biological product quality control
• Public health laboratories
• Research and teaching laboratories
• Third-party testing laboratories
• Microbial limit testing
• Sterility-related microbial monitoring
• Plate count analysis

The instrument can be used for colony counting on pour plates, spread plates, membrane filtration plates, spiral plates, microbial test films, and other culture formats.

Technical Specifications

Item	Specification
Product Name	Fully Automatic Colony Counter
Model	HD-C330
Main Function	Automatic colony imaging, colony recognition, colony counting, data analysis, and report output
Imaging Chamber	Fully enclosed steel-aluminum alloy dark chamber
Chamber Function	Eliminates ambient stray light, blocks UV leakage, and prevents dust entry
Illumination System	Fog diffuse reflection lighting, white transparent bottom lighting, compound suspended dark-field lighting
Imaging Light Source	High-density white and blue LED transmitted light
Light Source Adjustment	6-color adjustable illumination with single or combined lighting modes
UV Sterilization	254 nm UV lamp for internal chamber disinfection
Imaging Camera	High-definition CCD
Imaging Pixels	8.5 million pixels
Data Interface	USB 3.0
Minimum Resolution	≥0.02 mm
Applicable Plate Types	Pour plate, spread plate, membrane filtration plate, spiral plate, microbial test film
Counting Mode	Multiple preset intelligent counting modes
Complex Colony Analysis	Impurity removal, connected colony separation, regional counting
Statistical Information	Colony number, area, perimeter, equivalent diameter, dilution calculation
Measurement Functions	Built-in calibration, manual correction calibration, quick measurement, full-plate automatic measurement, multi-directional ruler measurement, manual precise measurement
Data Security	Multi-level permission control and anti-tampering protection
Data Record	Complete electronic sample information record
Data Output	Automatic electronic storage, PDF output, Excel output, printing
Standard Configuration	Colony counter main unit, colony analysis software, branded business desktop computer

Imaging System

Fully Enclosed Imaging Chamber

The HD-C330 uses a fully enclosed steel-aluminum alloy imaging chamber. This structure helps eliminate ambient stray light, block ultraviolet leakage, and prevent dust from entering the imaging area.

A stable imaging environment is important for colony recognition accuracy, especially when handling plates with low contrast, small colonies, transparent colonies, or complex background interference.

Full-Mode Illumination System

The illumination system integrates multiple lighting methods, including:

• Fog diffuse reflection illumination
• White transparent bottom illumination
• Compound suspended dark-field illumination
• Upper light source
• Lower light source
• Transmitted light source

These lighting modes allow the system to adapt to different plate types and colony appearances.

Six-Color Adjustable Lighting

The light source supports six-color adjustment and can be freely switched between single-color and combined illumination modes. This helps users obtain uniform Petri dish lighting and improve colony image quality under different sample conditions.

Fog Diffuse Reflection Design

The fog diffuse reflection system uses an LED array and nano optical reflection material to create surrounding fog-like illumination. This design helps reduce edge light spots on glass Petri dishes, preventing image artifacts from affecting software analysis.

UV Chamber Sterilization

The instrument is equipped with a 254 nm UV lamp, which can disinfect and sterilize the inside of the imaging chamber. This helps maintain a cleaner imaging environment for routine laboratory use.

High-Definition CCD Imaging

The system uses a high-definition CCD camera with 8.5 million imaging pixels. The USB 3.0 interface provides fast image transmission, and the minimum resolution can reach ≥0.02 mm, supporting accurate colony image acquisition and analysis.

Colony Analysis Module

Applicable Plate Types

The HD-C330 is suitable for different microbial culture formats, including:

• Pour plates
• Spread plates
• Membrane filtration plates
• Spiral plates
• Microbial test films

This makes the instrument suitable for routine colony counting in food, pharmaceutical, environmental, and research laboratories.

Intelligent Counting Modes

The software provides multiple preset intelligent counting modes. Users can quickly count colonies with one click without complex parameter setting.

This simplifies daily operation and helps reduce the trial-and-error process often required in manual image analysis.

Complex Colony Counting

For complex colony images, the software can analyze colonies based on:

• Colony size
• Colony color
• Colony morphology
• Colony area
• Colony shape characteristics

It can quickly remove impurities, automatically segment connected colonies, and count colonies within circular, square, or manually selected regions.

Statistical Information

The software can display and calculate multiple colony parameters, including:

• Colony number
• Colony area
• Colony perimeter
• Equivalent diameter
• Dilution factor conversion

Automatic dilution calculation helps reduce manual calculation errors and improves result consistency.

Measurement and Calibration Functions

The system supports multiple auxiliary functions, including:

• Built-in calibration
• Manual correction calibration
• One-click quick measurement
• Full-plate automatic measurement
• Multi-directional ruler measurement
• Manual precise measurement

These functions make the instrument suitable not only for counting but also for colony size analysis and image-based measurement tasks.

Data Security and Management

Multi-Level Permission Control

The system supports multi-level permission settings to help laboratories manage user access and operation authority. This prevents unauthorized data modification and supports data integrity management.

Anti-Tampering Protection

The software provides anti-tampering functions to help ensure that test data remain secure, complete, and authentic.

Complete Electronic Records

The system records complete sample information electronically, helping laboratories standardize sample traceability and data management.

Flexible Data Output

Test data and colony counting results can be:

• Automatically stored
• Printed
• Exported as PDF files
• Exported as Excel files

This supports daily laboratory reporting, quality control records, and audit documentation.

Standard Configuration

The HD-C330 standard configuration includes:

Item	Quantity
Colony Counter Main Unit	1 unit
Colony Analysis Software	1 set
Branded Business Desktop Computer	1 set

Operation Workflow

A typical HD-C330 workflow includes:

1. Prepare the culture plate or microbial test film
2. Place the sample into the enclosed imaging chamber
3. Select the appropriate lighting mode
4. Capture a high-resolution colony image
5. Choose the suitable intelligent counting mode
6. Software automatically identifies and counts colonies
7. Connected colonies and impurities are processed if needed
8. Dilution factor and statistical data are calculated
9. Results are reviewed and corrected if necessary
10. Data are stored, printed, or exported as PDF or Excel files

This workflow helps laboratories complete colony counting more efficiently and with better repeatability than manual counting.

Applicable Laboratories and Institutions

The HD-C330 Fully Automatic Colony Counter is suitable for:

• Food safety testing laboratories
• Pharmaceutical microbiology laboratories
• Biopharmaceutical quality control laboratories
• Environmental testing laboratories
• Water quality testing laboratories
• Public health laboratories
• Disease control laboratories
• Research institutes
• Universities and teaching laboratories
• Third-party testing laboratories
• Industrial quality control departments

Product Advantages

No Ambient Light Interference

The fully enclosed steel-aluminum alloy imaging chamber eliminates external light interference and provides a stable imaging environment.

Multiple Lighting Modes

The full-mode illumination system supports fog diffuse reflection, bottom transmitted light, and dark-field illumination, making it suitable for different Petri dishes and colony types.

Better Imaging for Glass Petri Dishes

The fog diffuse reflection design helps prevent edge light spots on glass plates, improving image quality and software recognition performance.

Intelligent Colony Recognition

The software uses intelligent image recognition algorithms to process complex colony images and reduce manual parameter adjustment.

Automatic Connected Colony Separation

Connected colonies can be automatically segmented, helping improve counting accuracy for dense or overlapping colony samples.

Flexible Regional Counting

The system can count colonies in full plates, circular areas, square areas, or manually selected regions.

High-Resolution Imaging

The 8.5-megapixel CCD camera and ≥0.02 mm minimum resolution support clear colony image acquisition.

Data Security and Traceability

Multi-level permissions, anti-tampering protection, electronic records, and flexible data output help laboratories manage data safely and efficiently.

Easy Report Output

Results can be stored, printed, or exported in PDF or Excel format, making data sharing and documentation easier.

Installation Requirements

The HD-C330 should be installed on a stable laboratory bench in a clean, dry, and well-ventilated environment. The laboratory should provide sufficient space for the main unit, computer workstation, sample operation, and data management.

The instrument should be placed away from strong vibration, corrosive gases, excessive dust, and direct sunlight. A stable power supply should be prepared according to the system configuration.

Professional installation and operator training are recommended to ensure correct software setup, imaging calibration, lighting adjustment, and daily operation.

Maintenance Requirements

Routine maintenance includes cleaning the imaging chamber, checking the light source, cleaning the sample stage, verifying camera imaging quality, maintaining the UV lamp, backing up data, and updating the software when required.

The chamber should be kept clean to prevent dust or residues from affecting image quality. If UV sterilization is used, users should follow laboratory safety procedures and avoid direct exposure to UV light.

Regular calibration and software verification are recommended to maintain stable counting performance.

Packaging and Export

The HD-C330 can be packed with protective materials to ensure safe transportation of the colony counter main unit, imaging system, software materials, and computer workstation.

For export projects, product configuration guidance, technical documents, operation manuals, installation support, software training, and after-sales service can be provided according to customer requirements.

Customization Service

The system can be configured according to different laboratory workflows, plate types, data management requirements, and reporting formats.

Customization options may include:

• Colony analysis software configuration
• Report format customization
• Data export format setting
• User permission management setup
• Computer workstation configuration
• Application-specific counting mode optimization
• Laboratory workflow integration

Product appearance, structure, performance, software functions, and technical parameters may change due to continuous product upgrades. Final configuration should be confirmed according to the latest product information.

Related Equipment and Applications

The HD-C330 can be used as part of a complete microbiology testing and laboratory automation solution. Related equipment and applications include:

• Automatic colony counter
• Microbial colony counter
• Colony analysis system
• Digital colony counting instrument
• Plate colony counter
• Membrane filtration colony counter
• Spiral plate colony counter
• Microbial test film colony counter
• Food microbiology testing system
• Pharmaceutical microbiology testing system
• Water quality microbiology testing
• Environmental microbiology analysis
• Microbial limit testing system

FAQ

1. What is the HD-C330 used for?

The HD-C330 is used for automatic colony imaging, intelligent colony recognition, colony counting, data analysis, and report output.

2. What plate types can it analyze?

It can analyze pour plates, spread plates, membrane filtration plates, spiral plates, microbial test films, and other common microbial culture formats.

3. Does the system require manual parameter setting?

The software provides multiple preset intelligent counting modes, allowing one-click colony counting without complex manual parameter adjustment.

4. Can it count connected colonies?

Yes. The software can automatically segment connected colonies and process complex colony images.

5. Can it remove impurities during counting?

Yes. The software can help exclude impurities based on colony size, color, morphology, and other image features.

6. What information does the software provide?

The software can display colony number, area, perimeter, equivalent diameter, and dilution calculation results.

7. What is the imaging resolution?

The system uses an 8.5-megapixel CCD camera, and the minimum resolution can reach ≥0.02 mm.

8. Does the instrument have UV sterilization?

Yes. The imaging chamber is equipped with a 254 nm UV lamp for internal disinfection.

9. How are results exported?

Results can be automatically stored, printed, or exported in PDF or Excel format.

10. What is included in the standard configuration?

The standard configuration includes one colony counter main unit, colony analysis software, and a branded business desktop computer.